Ming-Cheng Lin
Central Taiwan University of Science and Technology, Taiwan
Title: Neuroprotective mechanism of Antrodia Camphorata on the ischemic brain cortex is associated with reduced iron and copper level together with enhanced antioxidant enzyme activity
Biography
Biography: Ming-Cheng Lin
Abstract
Statement of the Problem: Recent investigation has demonstrated that Antrodia Camphorata (AC) has received a great deal of attention due to its beneficial effects such as anti-inflammation and anti-oxidation. Excessive transition metal level of iron (Fe) and copper (Cu) has been proposed to be harmful to the health. In this current experiment, we hypothesis that neuroprotective mechanisn of AC is associated with reducing level of Fe and Cu, and enhancing antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in the ischemic brain. Methodology & Theoretical Orientation: Male Sprague-Dawley rats were divided into control and AC-treated subject. Both groups were intraperitoneally injected with saline and AC for 14 days, respectively. On day 15, cerebral ischemic surgery was performed by occlusion of right middle cerebral artery (MCA) and right common carotid artery (CCA) for 1 hour. After completing surgery, rats were sacrificed and right brain cortex was isolated followed by homogenates. Supernatants were collected and ready for analysis of Fe and Cu level. Meanwhile, enzyme activity of SOD, CAT, GPX, and malondialdehyde (MDA) level were measured. Conclusion & Significance: Obtained data showed that level of Fe, Cu, and MDA was obviously declined (P < 0.05) in AC-treated subject. Conversely, a statistical (P < 0.05) enhancement of the enzyme activity of SOD, CAT, and GPX was found in AC-treated group. Based on our current findings, it seems likely to manifest the possibility here that neuroprotective mechanisms of AC are associated with declining Fe and Cu level together with enhancing enzyme activity of SOD, CAT, and GPX on the ischemic brain.