Nadia Mohd Effendy
Islamic Science University of Malaysia
Title: Labisia Pumila Regulates the Expressions of Bone-Related Genes and Pro-Inflammatory Cytokines in Postmenopausal Women
Biography
Biography: Nadia Mohd Effendy
Abstract
Background: Labisia pumila var. alata (LP) has shown potential as an anti-osteoporotic agent in recent years. The crude extract of LP was reported to reverse trabecular microarchitecture changes induced by ovariectomy. The mechanism is still unclear but it may be elucidated by examining the bone molecular pathways. This study aimed to evaluate the bone-related genes and pro-inflammatory cytokines expressions to provide a mechanistic overview on how the different LP extracts could prevent degenerative bone changes. Methods: Forty-eight female Sprague-Dawley rats were randomly divided into sham-operated (Sham), ovariectomized control (OVX), ovariectomized and given oestrogen at 64.5µg/kg (ERT), ovariectomized and given LP aqueous extract at 100 mg/kg (LPaq), ovariectomized and given LP methanol extract at 100 mg/kg (LPmet) and ovariectomized and given LP ethanol extract at 100 mg/kg (LPet) All treatments were given daily via oral gavages for nine weeks. Rats were then euthanized and femora dissected out for genes and cytokines expressions analysis. Results: Among the extracts, LPaq was found to produce the highest expressions of OPG, osteocalcin, osterix, osteonectin and ALP. LPaq was also shown to down-regulate the expressions of RANKL, MMP9, M-CSF as well as cytokine IL-1β. Conclusion: Aqueous extract is the best extract of LP which may exert anti-osteoporosis activity by regulating the bone-related genes and cytokines expressions.