Reshma A. Jain
The M. S. University of Baroda, India
Title: Optimization and evaluation of in-vivo and in-vitro immunostimulant activity of polyherbal formulations
Biography
Biography: Reshma A. Jain
Abstract
Immunomodulation deals with modifying immunity response using either natural or synthetic substances. To develop polyherbal formulation dose is the main factor to influence the process. The formulations are optimized for in-vitro antioxidant activity by applying factorial design. Factorial design, used to determine process variations, is applied for optimizing process parameters with reduced number of experiments. In current work, a four factor system consisting of the four herbal drugs such as Glycyrrhiza glabra root, Nelumbo nucifera leaves, aerial parts of Prunella vulgaris and fruits of Zizyphus jujuba and 24 runs were used for the optimization of formulations. The chosen dependent variable was EC50 (50% effective concentration). The lowest EC50 value (25.2 ± 1.27) of the batch indicates that 100mg dose of the all four plants give the best batch for the combination of all four plant extracts. Polyherbal formulations was screened for their in-vivo immunomodulatory activity using cyclophosphamide induced myelosupression models and in–vitro using NBT reduction test. Bone marrow suppression by cyclophosphamide was significant and was accompanied by lowered total WBC counts. Developed polyherbal formulations effectively increased the neutrophils’ functionality i.e. intracellular killing assayed by in-vitro NBT reduction test.