Farheen Fatima
University of Tabuk, KSA
Title: Impact of phytochemical-diallyl di sulphide (dads) on the biochemical components of the cell envelope of staphylococcus aureus
Biography
Biography: Farheen Fatima
Abstract
DiallylDisulphide (DADS) is a phytochemical found in the plants of the genus Allium. It is the principal organosulphuric component of distilled garlic oil. Many medicinal benefits like antimicrobial, antiviral and insecticidal are attributed this compound. However the biochemical explanation for such an action remains unexplored. This current study strives to explore the explanation for antimicrobial activity of the compound using the most common skin inhabitant Gram positive bacteria, commonly causing nosocomial infections- Staphylococcus aureus. The Minimum Inhibition Concentration(MIC), Minimum Bactericidal (MBC) Concentration of DADS, its effect on growth curve and time kill curve of S.aureushas been established. In the current study S.aureus was cultured in the broth containing 1/8 MIl analytic tests. The biochemical components focused on were of those abundantly present and those that contribute to the materializC of DADS. The staphylococcal cells were harvested; biochemical components were extracted and quantitated using appropriate biochemicaation of the staphylococcal cell envelope total lipids, total lipid phosphorous and total carbohydrates. The biochemical component extracts of S.aureustested revealed significant reduction in the total lipids (70%), total lipids phosphorous (50%) and total carbohydrates (34%). This reduction in the components may be attributed to the hypolipidemic and hypoglycemic properties of DADS and could further lead to changes in the anabolic processes. Since the cell envelope of the bacterial cell is primarily made up of lipids, phospholipids and peptidoglycan (a carbohydrate polymer). The evident reductions in the studied components indicate reflective impact of DADS on the cell envelope of S.aureus. This study can be extrapolated in innovation of a new phytochemical-antibiotic against the life threatening multidrug resistant human pathogens.