Babatunji Emmanuel Oyinloye
University of Zululand, South Africa
Title: The modulatory effect of methanol extract of Piper guineense in CCl4-induced hepatotoxicity in male rats
Biography
Biography: Babatunji Emmanuel Oyinloye
Abstract
The aim of this study was focused on investigating the possible protective effect of Piper guineense against CCl4-induced hepatotoxicity in animal-model. Thirty male Wistar-albino rats were divided into five groups as follows: control, CCl4, pre-treatment, post-treatment and standard drug. Hepatotoxicity was induced by administering oral dose of CCl4 (1.2 g/kg bw) 3 times a week for 3 weeks. In the modulatory experiment, Piper guineense (PG), (400 mg/kg bw) was administered by oral gavage for 14 days prior to the administration of CCl4 and simultaneously with CCl4 in the pre-treatment group; PG (400 mg/kg bw) was administered simultaneously with CCl4 in the post-treatment group while Livolin forte (20 mg/kg bw) was administered simultaneously with CCl4 in the standard drug group. Administration of CCl4 induces histopathological alteration in the liver with concomitant increased activities of serum hepatic marker enzymes associated with increased level of lipid peroxidation. Similarly, there was decrease in non-enzymatic (reduced glutathione; GSH) and enzymatic antioxidants (glutathione S-transferase; GST), superoxide dismutase (SOD) and catalase; CAT). Elevation in serum triglyceride (TG) and total cholesterol (TC) levels was noticed along with decreased level of serum total protein (TP). Treatment with PG 400 mg/kg bw exhibited excellent modulatory activity with respect to the different parameters studied by reversing all the above-mentioned biochemical changes significantly in all experimental animals. These results suggest that PG offered protection comparable to that of Livolin forte with better efficacy when pre-treated with 400 mg/kg bw 14 days prior to CCl4-exposure.
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