Sherif R. Abdel-Aal
National Organization for Drug Control and Research, Egypt
Title: Phytochemical study and biological activity of leaves and flowers of ipomoea carnea j. grown in egypt
Biography
Biography: Sherif R. Abdel-Aal
Abstract
Ipomoea carnea was recorded along canals, drains, road sides, railways, waste lands and fluid edges in the Nile Delta. GC/MS was used for qualitative and quantitative analyses of lipoidal content of the leaves and flowers of I. carnea. 32 Components representing 83.548 % were identified in the fatty acid methyl esters of the leaves of I. carnea while 23 components representing 84.424 % were identified in the fatty acid methyl esters of the flowers. On the other hand, 22 components were identified in the unsaponifiable matter of the leaves representing 83.398 %, while 25 components were identified in the unsaponifiable matter of the flowers representing 91.069 %. HPLC was used for assay of rutin in leaves and flowers ethanol extract of I. carnea. The leaves ethanol extract showed higher concentration of rutin (8.85 mg/g dry wt) than flower ethanol extract (2.72 mg/g dry wt). The total phenolic compounds, the in-vitro and in-vivo antioxidant properties and the antimicrobial activity against gram-positive and gram-negative bacteria, fungi and their minimum inhibitory concentration (MIC) were evaluated. The results showed that the ethanol extract of flower contained higher phenolic compounds than ethanol extract of leaves (9.331and 6.6348 mg (GAE)/g dry wt, respectively). The ethanol extract of leaves possessed higher DPPH radical scavenging activity than ethanol extract of flowers (IC50 = 1.608 and 4.861 mg/ml respectively). The flowers ethanol extract showed higher antibacterial activity in comparison with gentamicin and ampicillin against Eschirechia coli, Streptococcus pneumonia and Bacillis subtilis with MIC values (3.9 µg/ ml, 3.9µg/ ml and 0.98 µg/ ml) respectively. The leaves ethanol extract showed mild potent antifungal activity compared with amphotericin B against Aspergillus fumigatus with MIC value (7.81 µg/ ml). The extracts were tested for cytotoxic activity against liver, breast and colon carcinoma cell lines; leaves extract showed potent cytotoxic activity against breast carcinoma cell lines (IC50=7.4 µg/ml) while it showed mild cytotoxic effect on liver and colon carcinoma cell lines (IC50=23 and 35 µg/ml respectively) comparing to doxorubicin while the flower extract showed only cytotoxic activity against breast carcinoma cell lines (IC50=44.2 µg/ml).