Foluso Osunsanami Oluwagbemiga
University of Zululand, South Africa
Title: Antiplatelet aggregation and cytotoxicity activity of betulinic acid and its acetyl derivative from Melaleuca bracteates var. revolution gold
Biography
Biography: Foluso Osunsanami Oluwagbemiga
Abstract
Platelet dysfunctions are implicated in cardiovascular diseases. Management of abnormal Platelet aggregations with natural products is a promising approach to the treatment of cardiovascular diseases. In this study, betulinic acid (BA) and its acetyl derivative (3-β acetylbetulinic acid) (BAA) from Melaleuca bracteata var. revolution gold were investigated for their antiplatelet aggregation and cytotoxicity. BA was isolated from Melaleuca bracetata by column chromatography and some portion of BA were used to synthesize BAA. The antiplatelet aggregation activity of the compounds was separately evaluated on collagen, ADP, thrombin and epinephrine to induce rat platelet aggregations. The MTT cytotoxicity assay was used to determine the cytotoxic effect of the compounds against human embryonic kidney (HEK293) and hepatocellular carcinoma (HEPG2) cell lines. BA and BAA exhibited significant (p<0.05) dose dependent antiplatelet aggregation activity. BA and BAA showed the highest platelet aggregation inhibition on epinephrine induced platelet aggregation with IC50 values 0.78 mg/ml and 0.85 mg/ml respectively. BA and BAA showed less cytotoxicity effect on both HEK293 cell (IC50 1027 µg/ml and 1051 µg/ml respectively) and HEPG2 cells (IC50 448 mg/ml and 672 mg/ml respectively). The results suggest that the compounds could serve as template for synthesis of new antiplatelet drugs Platelets.
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