Day 1 :
Sukul Institute of Homeopathic Research, India
Anirban Sukul has completed his PhD from Visva-Bharati University and Research Associate-ship from same university. He is the Director of Sukul Institute of Homeopathic Research, Kolkata, India. He has published 22 papers in international journals and authored a book published by Kluwer Academic Publishers, Netherlands. He is conferred the Junior Scientist Award, Life Time Achievement Award, Gold Medal Award (UK), Man of Excellence Award, etc. He is in Board of Editors in many reputed international journals published from London, USA. He is also a Member of Research Committee, LMHI, Germany.
Two pairs of rows of cowpea plants Vigna unguiculata (L) Walp, were grown in earthen pots, each row containing 10 plants. Plants in each pair were connected by polythene tubes filled in water, the ends being dipped into water of two beakers. In each beaker, a mature leaf was immersed. Plants in one row of a pair were given heat stress through hot water while the corresponding water connected row of the same pair remained unstressed. Plants in one row of the second pair treated with Cantharis 200C, a homeopathy potency, used for the treatment for burns. The corresponding water connected row of this pair remained untreated. Another single row of plants served as the unstressed and untreated control. After a fixed time leaves of all the plants were harvested and homogenized. Leaf proteins of the plants in each row were separated by Fast Protein Liquid Chromatography (FPLC). Leaf protein profile of the heat stressed plants showed similarity with that of unstressed but water connected plants. Cantharis-treated plants and the corresponding untreated but water connected ones showed similarity in the leaf protein profile. Leaf protein profile of the control plants was different from that of the two groups. It appears that an external stimulus to a plant brings about a change in the water structure in the plant which is transmitted through the Global Molecular Network (GMN) of water connecting the two plants.
Thammasat University, Thailand
Sophida Sukprasert has completed her PhD in field of Biochemistry from Khon Kaen University, Thailand. Presently she is a Lecturer and Researcher at Division of Integrative Medicine, Chulabhorn International College of Medicine, Thammasat University since 2013. She is interested in the herbal medicines which possess antidote and anti-diabetic properties.
The leaves and stems of Lysiphyllum strychnifolium (Craib) A. Schmitz (Fabaceae family) have been traditionally used in Thailand for detoxification and to treat pesticide poisoning in humans. To uncover novel uses of L. strychnifolium, the possible antiviral properties against avian influenza virus A, strain H5N1, were explored in this study. The ethanolic extracts of L. strychnifolium leaves and stems showed good inhibitory activities against H5N1 which have never been previously reported of this plant, while the inhibition derived from aqueous extracts was not observed. Thereafter, the anti-neuraminidase activities of ethanolic extracts were evaluated using fluorometric determination via a MUNANA-based enzyme inhibition assay. Both stems and leaves ethanolic extracts showed good inhibitory activities against neuraminidase of Influenza A H5N1 with IC50 value of 55.30 and 70.00 μg/mL, respectively. Moreover, anti-bacterial activities of aqueous and ethanolic extracts of L. strychnifolium leaves and stems were tested using the disc diffusion method. All extracts showed broad antibacterial activities against both Gram positive and Gram negative bacterial strains. Phytochemical constituents of all extracts were identified through Gas Chromatography-Mass Spectrometry (GC-MS) and revealed some compounds such as Methyl-phydrozybenzoate; Mome inositol; n-Hexadecanoic acid; Tetradecanamide; (Z)-9-Octadecanamide; 1,2,3,- Benzenetriol; Methylparaben; 4-(4-Hydroxyphenyl)-2-butanone; n-Hexadecanoic acid; Ethyl hexadecanoate; Phytol; (Z)-9-Octadecanoic acid and Octadecanoic acid. This is the first report of neuraminidase inhibitor against Influenza A H5N1 and antibacterial activities derived from L. strychnifolium extracts. It could be suggested that this plant is an alternative source for treatment of influenza viruses and antibiotic agent.